| 质粒名称: | AdEasier-1 cells (strain) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 16399 |
| 存储实验室: | Bert Vogelstein |
|
载体骨架基本信息 |
|
| 载体名称: | n/a |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 33477 bp |
| 载体类型: | 大肠杆菌 |
| 载体抗性: | Ampicillin and Streptomycin |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | AdEasier-1 bacterial cells、AdEasier Cells、BJAdEasy Cells |
| 物种来源: | E. coli |
| 插入大小: | 951 bp |
| GenBank编号 | AY370909 |
| 启动子: | CMV |
| 5’测序引物 | n/a |
|
文献引用方法 |
|
| 材料和方法部分: |
AdEasier-1 cells (strain) was a gift from Bert Vogelstein (Addgene plasmid # 16399)
|
| 参考文献部分: | A simplified system for generating recombinant adenoviruses. He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B. Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. 10.1073/pnas.95.5.2509 PubMed 9482916 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS AdEasier-1 cells (strain) 33477 bp DNA circular UNA 25-AUG-2024
DEFINITION natural circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE natural DNA sequence
ORGANISM unspecified
REFERENCE 1 (bases 1 to 33477)
AUTHORS He TC, Zhou S, da Costa LT, Yu J, Kinzler KW, Vogelstein B
TITLE A simplified system for generating recombinant adenoviruses.
JOURNAL Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14.
PUBMED 9482916
REFERENCE 2 (bases 1 to 33477)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.baofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl
Acad Sci U S A. 1998 Mar 3. 95(5):2509-14."
FEATURES Location/Qualifiers
source 1..33477
/mol_type="genomic DNA"
/organism="unspecified"
primer_bind complement(19..38)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 179..201
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(195..386)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
repeat_region 1677..1779
/label=ITR
/note="inverted terminal repeat of human adenovirus
serotype 5"
regulatory 13240..13249
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 20721..20732
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
misc_feature 27825..27928
/label=Ad SA
/note="adenovirus major late transcript splice acceptor
(Friedrich and Soriano, 1991)"
promoter 31520..31624
/gene="bla"
/label=AmpR promoter
CDS 31625..32485
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(31843..31862)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 32656..33244
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 33145..33164
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 33398..33415
/label=L4440
/note="L4440 vector, forward primer"
misc_feature join(33430..33477,1..93)
/label=bom
/note="basis of mobility region from pBR322"
ORIGIN
1 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
61 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
121 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
181 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg
241 taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc
301 agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta
361 agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg
421 ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa
481 catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac
541 cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca
601 cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac
661 ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag
721 gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc
781 tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc
841 atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag
901 atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc
961 aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt
1021 ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa
1081 ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat
1141 aaatcgccgt gacgatcagc ggtccaatga tcgaagttag gctggtaaga gccgcgagcg
1201 atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg
1261 cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc
1321 gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg
1381 cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt
1441 gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt
1501 cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga
1561 agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg
1621 ggttgaaggc tctcaagggc atcggtcgag acgtaggatc catgcatgtt aattaacatc
1681 atcaataata taccttattt tggattgaag ccaatatgat aatgaggggg tggagtttgt
1741 gacgtggcgc ggggcgtggg aacggggcgg gtgacgtagg ttttagggcg gagtaacttg
1801 tatgtgttgg gaattgtagt tttcttaaaa tgggaagtga cgtaacgtgg gaaaacggaa
1861 gtgacgattt gaggaagttg tgggtttttt ggctttcgtt tctgggcgta ggttcgcgtg
1921 cggttttctg ggtgtttttt gtggacttta accgttacgt cattttttag tcctatatat
1981 actcgctctg cacttggccc ttttttacac tgtgactgat tgagctggtg ccgtgtcgag
2041 tggtgttttt ttaataggtt ttctttttta ctggtaaggc tgactgttat ggctgccgct
2101 gtggaagcgc tgtatgttgt tctggagcgg gagggtgcta ttttgcctag gcaggagggt
2161 ttttcaggtg tttatgtgtt tttctctcct attaattttg ttatacctcc tatgggggct
2221 gtaatgttgt ctctacgcct gcgggtatgt attcccccgg gctatttcgg tcgcttttta
2281 gcactgaccg atgtgaatca acctgatgtg tttaccgagt cttacattat gactccggac
2341 atgaccgagg agctgtcggt ggtgcttttt aatcacggtg accagttttt ttacggtcac
2401 gccggcatgg ccgtagtccg tcttatgctt ataagggttg tttttcctgt tgtaagacag
2461 gcttctaatg tttaaatgtt tttttgttat tttattttgt gtttatgcag aaacccgcag
2521 acatgtttga gagaaaaatg gtgtcttttt ctgtggtggt tccggagctt acctgccttt
2581 atctgcatga gcatgactac gatgtgcttt cttttttgcg cgaggctttg cctgattttt
2641 tgagcagcac cttgcatttt atatcgccgc ccatgcaaca agcttacatc ggggctacgc
2701 tggttagcat agctccgagt atgcgtgtca taatcagtgt gggttctttt gtcatggttc
2761 ctggcgggga agtggccgcg ctggtccgtg cagacctgca cgattatgtt cagctggccc
2821 tgcgaaggga cctacgggat cgcggtattt ttgttaatgt tccgcttttg aatcttatac
2881 aggtctgtga ggaacctgaa tttttgcaat catgattcgc tgcttgaggc tgaaggtgga
2941 gggcgctctg gagcagattt ttacaatggc cggacttaat attcgggatt tgcttagaga
3001 tatattgaga aggtggcgag atgagaatta tttgggcatg gttgaaggtg ctggaatgtt
3061 tatagaggag attcaccctg aagggtttag cctttacgtc cacttggacg tgagggccgt
3121 ttgccttttg gaagccattg tgcaacatct tacaaatgcc attatctgtt ctttggctgt
3181 agagtttgac cacgccaccg gaggggagcg cgttcactta atagatcttc attttgaggt
3241 tttggataat cttttggaat aaaaaaaaaa acatggttct tccagctctt cccgctcctc
3301 ccgtgtgtga ctcgcagaac gaatgtgtag gttggctggg tgtggcttat tctgcggtgg
3361 tggatgttat cagggcagcg gcgcatgaag gagtttacat agaacccgaa gccagggggc
3421 gcctggatgc tttgagagag tggatatact acaactacta cacagagcga tctaagcggc
3481 gagaccggag acgcagatct gtttgtcacg cccgcacctg gttttgcttc aggaaatatg
3541 actacgtccg gcgttccatt tggcatgaca ctacgaccaa cacgatctcg gttgtctcgg
3601 cgcactccgt acagtaggga tcgtctacct ccttttgaga cagaaacccg cgctaccata
3661 ctggaggatc atccgctgct gcccgaatgt aacactttga caatgcacaa cgtgagttac
3721 gtgcgaggtc ttccctgcag tgtgggattt acgctgattc aggaatgggt tgttccctgg
3781 gatatggttc taacgcggga ggagcttgta atcctgagga agtgtatgca cgtgtgcctg
3841 tgttgtgcca acattgatat catgacgagc atgatgatcc atggttacga gtcctgggct
3901 ctccactgtc attgttccag tcccggttcc ctgcagtgta tagccggcgg gcaggttttg
3961 gccagctggt ttaggatggt ggtggatggc gccatgttta atcagaggtt tatatggtac
4021 cgggaggtgg tgaattacaa catgccaaaa gaggtaatgt ttatgtccag cgtgtttatg
4081 aggggtcgcc acttaatcta cctgcgcttg tggtatgatg gccacgtggg ttctgtggtc
4141 cccgccatga gctttggata cagcgccttg cactgtggga ttttgaacaa tattgtggtg
4201 ctgtgctgca gttactgtgc tgatttaagt gagatcaggg tgcgctgctg tgcccggagg
4261 acaaggcgcc ttatgctgcg ggcggtgcga atcatcgctg aggagaccac tgccatgttg
4321 tattcctgca ggacggagcg gcggcggcag cagtttattc gcgcgctgct gcagcaccac
4381 cgccctatcc tgatgcacga ttatgactct acccccatgt aggcgtggac ttctccttcg
4441 ccgcccgtta agcaaccgca agttggacag cagcctgtgg ctcagcagct ggacagcgac
4501 atgaacttaa gtgagctgcc cggggagttt attaatatca ctgatgagcg tttggctcga
4561 caggaaaccg tgtggaatat aacacctaag aatatgtctg ttacccatga tatgatgctt
4621 tttaaggcca gccggggaga aaggactgtg tactctgtgt gttgggaggg aggtggcagg
4681 ttgaatacta gggttctgtg agtttgatta aggtacggtg atctgtataa gctatgtggt
4741 ggtggggcta tactactgaa tgaaaaatga cttgaaattt tctgcaattg aaaaataaac
4801 acgttgaaac ataacacaaa cgattcttta ttcttgggca atgtatgaaa aagtgtaaga
4861 ggatgtggca aatatttcat taatgtagtt gtggccagac cagtcccatg aaaatgacat
4921 agagtatgca cttggagttg tgtctcctgt ttcctgtgta ccgtttagtg taatggttag
4981 tgttacaggt ttagttttgt ctccgtttaa gtaaacttga ctgacaatgt tacttttggc
5041 agttttaccg tgagattttg gataagctga taggttaggc ataaatccaa cagcgtttgt
5101 ataggctgtg ccttcagtaa gatctccatt tctaaagttc caatattctg ggtccaggaa
5161 ggaattgttt agtagcactc cattttcgtc aaatcttata ataagatgag cactttgaac
5221 tgttccagat attggagcca aactgccttt aacagccaaa actgaaactg tagcaagtat
5281 ttgactgcca cattttgtta agaccaaagt gagtttagca tctttctctg catttagtct
5341 acagttagga gatggagctg gtgtggtcca caaagttagc ttatcattat ttttgtttcc
5401 tactgtaatg gcacctgtgc tgtcaaaact aaggccagtt cctagtttag gaaccatagc
5461 cttgtttgaa tcaaattcta ggccatggcc aatttttgtt ttgaggggat ttgtgtttgg
5521 tgcattaggt gaaccaaatt caagcccatc tcctgcatta atggctatgg ctgtagcgtc
5581 aaacatcaac cccttggcag tgcttaggtt aacctcaagc tttttggaat tgtttgaagc
5641 tgtaaacaag taaaggcctt tgttgtagtt aatatccaag ttgtgggctg agtttataaa
5701 aagagggccc tgtcctagtc ttagatttag ttggttttga gcatcaaacg gataactaac
5761 atcaagtata aggcgtctgt tttgagaatc aatccttagt cctcctgcta cattaagttg
5821 catattgcct tgtgaatcaa aacccaaggc tccagtaact ttagtttgca aggaagtatt
5881 attaatagtc acacctggac cagttgctac ggtcaaagtg tttaggtcgt ctgttacatg
5941 caaaggagcc ccgtacttta gtcctagttt tccattttgt gtataaatgg gctctttcaa
6001 gtcaatgccc aagctaccag tggcagtagt tagagggggt gaggcagtga tagtaagggt
6061 actgctatcg gtggtggtga gggggcctga tgtttgcagg gctagctttc cttctgacac
6121 tgtgaggggt ccttgggtgg caatgctaag tttggagtcg tgcacggtta gcggggcctg
6181 tgattgcatg gtgagtgtgt tgcccgcgac cattagaggt gcggcggcag ccacagttag
6241 ggcttctgag gtaactgtga ggggtgcaga tatttccagg tttatgtttg acttggtttt
6301 tttgagaggt gggctcacag tggttacatt ttgggaggta aggttgccgg cctcgtccag
6361 agagaggccg ttgcccattt tgagcgcaag catgccattg gaggtaacta gaggttcgga
6421 taggcgcaaa gagagtaccc cagggggact ctcttgaaac ccattggggg atacaaaggg
6481 aggagtaaga aaaggcacag ttggaggacc ggtttccgtg tcatatggat acacggggtt
6541 gaaggtatct tcagacggtc ttgcgcgctt catctgcaac aacatgaaga tagtgggtgc
6601 ggatggacag gaacaggagg aaactgacat tccatttaga ttgtggagaa agtttgcagc
6661 caggaggaag ctgcaatacc agagctggga ggagggcaag gaggtgctgc tgaataaact
6721 ggacagaaat ttgctaactg attttaagta agtgatgctt tattattttt ttttattagt
6781 taaagggaat aagatccccg ggtactctag ttaatcgact taggcgacct gacattaact
6841 agaggatctt gatgtaatcc agggttagga cagttgcaaa tcacagtgag aacacagggt
6901 cccctgtccc gctcaactag cagggggcgc tgggtaaact cccgaatcag gctacgggca
6961 agctctccct gggcggtaag ccggacgccg tgcgccgggc cctcgatatg atcctcgggc
7021 aattcaaagt agcaaaactc accggagtcg cgggcaaagc acttgtggcg gcgacagtgg
7081 accaggtgtt tcaggcgcag ttgctctgcc tctccactta acattcagtc gtagccgtcc
7141 gccgagtcct ttaccgcgtc aaagttagga ataaattgat ccggatagtg gccgggaggt
7201 cccgagaagg ggttaaagta gaccgatggc acaaactcct caataaattg cagagttcca
7261 atgcctccag agcgcggctc agaggacgag gtctgcagag ttaggattgc ctgacgaggc
7321 gtgaatgaag agcggccggc gccgccgatc tgaaatgtcc cgtccggacg gagaccaagc
7381 gaggagctca ccgactcgtc gttgagctga atacctcgcc ctctgattgt caggtgagtt
7441 ataccctgcc cgggcgaccg caccctgtga cgaaagccgc ccgcaagctg cgcccctgag
7501 ttagtcatct gaacttcggc ctgggcgtct ctgggaagta ccacagtggt gggagcggga
7561 ctttcctggt acaccagggc agcgggccaa ctacggggat taaggttatt acgaggtgtg
7621 gtggtaatag ccgcctgttc caggagaatt cggtttcggt gggcgcgtat tccgttgacc
7681 cgggatatca tgtggggtcc cgcgctcatg tagtttattc gggttgagta gtcttgggca
7741 gctccagccg caagtcccat ttgtggctgg taactccaca tgtagggcgt gggaatttcc
7801 ttgctcataa tggcgctgac aacaggtgct ggcgccgggt gtggccgctg gagatgacgt
7861 agttttcgcg cttaaatttg agaaagggcg cgaaactagt ccttaagagt cagcgcgcag
7921 tatttactga agagagcctc cgcgtcttcc agcgtgcgcc gaagctgatc ttcgcttttg
7981 tgatacaggc agctgcgggt gagggatcgc agagacctgt tttttatttt cagctcttgt
8041 tcttggcccc tgctctgttg aaatatagca tacagagtgg gaaaaatcct gtttctaagc
8101 tcgcgggtcg atacgggttc gttgggcgcc agacgcagcg ctcctcctcc tgctgctgcc
8161 gccgctgtgg atttcttggg ctttgtcaga gtcttgctat ccggtcgcct ttgcttctgt
8221 gtggccgctg ctgttgctgc cgctgccgcc ggtgcagtat gggctgtaga gatgacggta
8281 gtaatgcagg atgttacggg ggaaggccac gccgtgatgg tagagaagaa agcggcgggc
8341 gaaggagatg ttgcccccac agtcttgcaa gcaagcaact atggcgttct tgtgcccgcg
8401 ccatgagcgg tagccttggc gctgttgttg ctcttgggct aacggcggcg gctgcttgga
8461 cttaccggcc ctggttccag tggtgtccca tctacggttg ggtcggcgaa cgggcagtgc
8521 cggcggcgcc tgaggagcgg aggttgtagc catgctggaa ccggttgccg atttctgggg
8581 cgccggcgag gggaatgcga ccgagggtga cggtgtttcg tctgacacct cttcgacctc
8641 ggaagcttcc tcgtctaggc tctcccagtc ttccatcatg tcctcctcct cctcgtccaa
8701 aacctcctct gcctgactgt cccagtattc ctcctcgtcc gtgggtggcg gcggcagctg
8761 cagcttcttt ttgggtgcca tcctgggaag caagggcccg cggctgctgc tgatagggct
8821 gcggcggcgg ggggattggg ttgagctcct cgccggactg ggggtccaag taaacccccc
8881 gtccctttcg tagcagaaac tcttggcggg ctttgttgat ggcttgcaat tggccaagaa
8941 tgtggccctg ggtaatgacg caggcggtaa gctccgcatt aggcgggcgg gattggtctt
9001 cgtagaacct aatctcgtgg gcgtggtagt cctcaggtac aaatttgcga aggtaagccg
9061 acgtccacag ccccggagtg agtttcaacc ccggagccgc ggacttttcg tcaggcgagg
9121 gaccctgcag ctcaaaggta ccgataattt gactttcgtt aagcagctgc gaattgcaaa
9181 ccagggagcg gtgcggggtg cataggttgc agcgacagtg acactccagt agaccgtcac
9241 cgctcacgtc ttccattatg tcagagtggt aggcaaggta gttggctagc tgcagaaggt
9301 agcagtggcc ccaaagcggc ggagggcatt cgcggtactt aatgggcaca aagtcgctag
9361 gaagtgcaca gcaggtggcg ggcaagattc ctgagcgctc taggataaag ttcctaaagt
9421 tctgcaacat gctttgactg gtgaagtctg gcagaccctg ttgcagggtt ttaagcaggc
9481 gttcggggaa aatgatgtcc gccaggtgcg cggccacgga gcgctcgttg aaggccgtcc
9541 ataggtcctt caagttttgc tttagcagtt tctgcagctc cttgaggttg cactcctcca
9601 agcactgctg ccaaacgccc atggccgtct gccaggtgta gcatagaaat aagtaaacgc
9661 agtcgcggac gtagtcgcgg cgcgcctcgc ccttgagcgt ggaatgaagc acgttttgcc
9721 caaggcggtt ttcgtgcaaa attccaaggt aggagaccag gttgcagagc tccacgttgg
9781 agatcttgca ggcctggcgt acgtagccct gtcgaaaggt gtagtgcaat gtttcctcta
9841 gcttgcgctg catctccggg tcagcaaaga accgctgcat gcactcaagc tccacggtaa
9901 cgagcactgc ggccatcatt agtttgcgtc gctcctccaa gtcggcaggc tcgcgcgttt
9961 gaagccagcg cgctagctgc tcgtcgccaa ctgcgggtag gccctcctct gtttgttctt
10021 gcaaatttgc atccctctcc aggggctgcg cacggcgcac gatcagctca ctcatgactg
10081 tgctcatgac cttggggggt aggttaagtg ccgggtaggc aaagtgggtg acctcgatgc
10141 tgcgttttag tacggctagg cgcgcgttgt caccctcgag ttccaccaac actccagagt
10201 gactttcatt ttcgctgttt tcctgttgca gagcgtttgc cgcgcgcttc tcgtcgcgtc
10261 caagaccctc aaagattttt ggcacttcgt tgagcgaggc gatatcaggt atgacagcgc
10321 cctgccgcaa ggccagctgc ttgtccgctc ggctgcggtt ggcacggcag gataggggta
10381 tcttgcagtt ttggaaaaag atgtgatagg tggcaagcac ctctggcacg gcaaatacgg
10441 ggtagaagtt gaggcgcggg ttgggctcgc atgtgccgtt ttcttggcgt ttggggggta
10501 cgcgcggtga gaataggtgg cgttcgtagg caaggctgac atccgctatg gcgaggggca
10561 catcgctgcg ctcttgcaac gcgtcgcaga taatggcgca ctggcgctgc agatgcttca
10621 acagcacgtc gtctcccaca tctaggtagt cgccatgcct ttcgtccccc cgcccgactt
10681 gttcctcgtt tgcctctgcg ttgtcctggt cttgcttttt atcctctgtt ggtactgagc
10741 ggtcctcgtc gtcttcgctt acaaaacctg ggtcctgctc gataatcact tcctcctcct
10801 caagcggggg tgcctcgacg gggaaggtgg taggcgcgtt ggcggcatcg gtggaggcgg
10861 tggtggcgaa ctcagagggg gcggttaggc tgtccttctt ctcgactgac tccatgatct
10921 ttttctgcct ataggagaag gaaatggcca gtcgggaaga ggagcagcgc gaaaccaccc
10981 ccgagcgcgg acgcggtgcg gcgcgacgtc ccccaaccat ggaggacgtg tcgtccccgt
11041 ccccgtcgcc gccgcctccc cgggcgcccc caaaaaagcg gatgaggcgg cgtatcgagt
11101 ccgaggacga ggaagactca tcacaagacg cgctggtgcc gcgcacaccc agcccgcggc
11161 catcgacctc ggcggcggat ttggccattg cgcccaagaa gaaaaagaag cgcccttctc
11221 ccaagcccga gcgcccgcca tcaccagagg taatcgtgga cagcgaggaa gaaagagaag
11281 atgtggcgct acaaatggtg ggtttcagca acccaccggt gctaatcaag catggcaaag
11341 gaggtaagcg cacagtgcgg cggctgaatg aagacgaccc agtggcgcgt ggtatgcgga
11401 cgcaagagga agaggaagag cccagcgaag cggaaagtga aattacggtg atgaacccgc
11461 tgagtgtgcc gatcgtgtct gcgtgggaga agggcatgga ggctgcgcgc gcgctgatgg
11521 acaagtacca cgtggataac gatctaaagg cgaacttcaa actactgcct gaccaagtgg
11581 aagctctggc ggccgtatgc aagacctggc tgaacgagga gcaccgcggg ttgcagctga
11641 ccttcaccag caacaagacc tttgtgacga tgatggggcg attcctgcag gcgtacctgc
11701 agtcgtttgc agaggtgacc tacaagcatc acgagcccac gggctgcgcg ttgtggctgc
11761 accgctgcgc tgagatcgaa ggcgagctta agtgtctaca cggaagcatt atgataaata
11821 aggagcacgt gattgaaatg gatgtgacga gcgaaaacgg gcagcgcgcg ctgaaggagc
11881 agtctagcaa ggccaagatc gtgaagaacc ggtggggccg aaatgtggtg cagatctcca
11941 acaccgacgc aaggtgctgc gtgcacgacg cggcctgtcc ggccaatcag ttttccggca
12001 agtcttgcgg catgttcttc tctgaaggcg caaaggctca ggtggctttt aagcagatca
12061 aggcttttat gcaggcgctg tatcctaacg cccagaccgg gcacggtcac cttttgatgc
12121 cactacggtg cgagtgcaac tcaaagcctg ggcacgcgcc ctttttggga aggcagctac
12181 caaagttgac tccgttcgcc ctgagcaacg cggaggacct ggacgcggat ctgatctccg
12241 acaagagcgt gctggccagc gtgcaccacc cggcgctgat agtgttccag tgctgcaacc
12301 ctgtgtatcg caactcgcgc gcgcagggcg gaggccccaa ctgcgacttc aagatatcgg
12361 cgcccgacct gctaaacgcg ttggtgatgg tgcgcagcct gtggagtgaa aacttcaccg
12421 agctgccgcg gatggttgtg cctgagttta agtggagcac taaacaccag tatcgcaacg
12481 tgtccctgcc agtggcgcat agcgatgcgc ggcagaaccc ctttgatttt taaacggcgc
12541 agacggcaag ggtgggggta aataatcacc cgagagtgta caaataaaag catttgcctt
12601 tattgaaagt gtctctagta cattattttt acatgttttt caagtgacaa aaagaagtgg
12661 cgctcctaat ctgcgcactg tggctgcgga agtagggcga gtggcgctcc aggaagctgt
12721 agagctgttc ctggttgcga cgcagggtgg gctgtacctg gggactgttg agcatggagt
12781 tgggtacccc ggtaataagg ttcatggtgg ggttgtgatc catgggagtt tggggccagt
12841 tggcaaaggc gtggagaaac atgcagcaga atagtccaca ggcggccgag ttgggcccct
12901 gtacgctttg ggtggacttt tccagcgtta tacagcggtc gggggaagaa gcaatggcgc
12961 tacggcgcag gagtgactcg tactcaaact ggtaaacctg cttgagtcgc tggtcagaaa
13021 agccaaaggg ctcaaagagg tagcatgttt ttgagtgcgg gttccaggca aaggccatcc
13081 agtgtacgcc cccagtctcg cgaccggccg tattgactat ggcgcaggcg agcttgtgtg
13141 gagaaacaaa gcctggaaag cgcttgtcat aggtgcccaa aaaatatggc ccacaaccaa
13201 gatctttgac aatggctttc agttcctgct cactggagcc catggcggca gctgttgttg
13261 atgttgcttg cttctttatg ttgtggcgtt gccggccgag aagggcgtgc gcaggtacac
13321 ggtttcgatg acgccgcggt gcggctggtg cacacggacc acgtcaaaga cttcaaacaa
13381 aacataaaga agggtgggct cgtccatggg atccacctca aaagtcatgt ctagcgcgtg
13441 ggcggagttg gcgtagagaa ggttttggcc caggtctgtg agtgcgccca tggacataaa
13501 gttactggag aatgggatgc gccaaagggt gcgatcgcaa agaaactttt tctgggtaat
13561 gctgtcaact gcggtcttgc ctataagcgg ataggggaag ttagcagggt aggcctgtcc
13621 ttcgcgcatg gtgggggcaa ggtagccaac aaatccagag ttgttgtgtt ggtgtaggat
13681 gcccacctgt tggtagtcct tgtatttagt atcatccacc acctgacggc tcatgggctg
13741 gaagtttcta aagaaggagt acatgcggtc cttgtagctc tctgggatat agaagccctg
13801 gtagccaatg ttatagttag ctagcatttg taccaggaac cagtctttgg tcatgttaca
13861 ctgggcaacg ttgtaaccct ccccgtcaac tgagcgctta atttcaaact cgttgggggt
13921 aagcaggcgg tcattgccag gccagctgac agaagagtca aaggtaatgg ccaccttctt
13981 aaaggtgtgg ttgaggtaaa aggttccatc taggtagggt atagagccag agtaggtgta
14041 ataagggtcg tagcccgagc ccagtgatgg ggtttcctta gtcttaaggc gcgtgaaggc
14101 ccagccgcgg aaagccgccc agttgcggga ggggatggat atgggcacgt tggtagcgtt
14161 ggcgggtata gggtagagca tgttggcggc ggagagatag tcgttaaagg actggtcgtt
14221 ggtgtcgttt ctaagcatgg cctcaagcgt ggaggcggtg ttgtgggcca tggggaagaa
14281 ggtggcgtaa aggcaaatgc tatcaaactt aatgctggct ccgtcaaccc ttaggtcatt
14341 tcctagggag ctctgcagaa ccatgttaac atccttcctg aagttccact cgtaggtgta
14401 tgagcccggc aggagaagga ggtttttaat ggcaaagaac ttctgaggca cctggatgtg
14461 gaagggcaca tagcgaccat tgcccagcaa cattgagcgg tagcgcaggc cagcattgcg
14521 gtggtggtta aatgggttga cgttgtccat atagtcaagg gaccagcgtg ctccaaggtt
14581 aatgtagcag tccactagcc cgggagccac cactcgcttg ttcatgtagt cgtaggtgtt
14641 tgggttatca gaaattttta cgttggaagg actgtacttt agcttgtcgg gcaaatacag
14701 cgctatgttg gagtacagga aatttctcca caggttggca tttagattga tttccatggc
14761 aaaattattt ccaactctta tttcattttt atctgaaaat tctgtagcat ctttttccca
14821 tccattttcc tgacctgttt taggttttac cttggtaaga gtctctgtat taatcacacc
14881 tcccagtgga aagcagtaat ttggaagttc atcttcagtt ccatgatttt caataattct
14941 aacatctgga tcatagctgt caacagcctg attccacata gaaaagtacc tggttctatc
15001 accaatggaa tcaagcaaaa gctggtatga aagctctgtg tttctgtctt gcaaatctac
15061 aacagcattc aactgcgatg cttggcccgc cagaacaccc atattacccg tgctgttgta
15121 atacattaga ccaataaaat tgtccctaaa agcaatgtaa ttaggcctgt tgggcataga
15181 ttgttggccc attagttctc gtgagttacc ttccttaata gtgggcatgt aagaaatatg
15241 agtgtctggg gtttctatat ctacatcttc actgtacaat accactttag gagtcaagtt
15301 atcaccattg cctgcggctg cctcagtagt tgagaaaaat tgcatttcca cttgactttc
15361 tagctttcca ttttgttgct ttacaagaat gccttgccct ccattttcat ttgtgggttt
15421 tgcatatgaa ccgtaacatg gtttcattgg ggtagtcttt tttaggactc tcccagctgc
15481 atgattaatt tctgtttcgt accactgaga ttctcctatt tgaggttcag gttgaaatgt
15541 tttatcggca tatttaggtg tttgaccttc gacacctatt tgaataccct cctttgtaat
15601 atttatacca gaataaggcg cctgcccaaa tacgtgagtt ttttgctgct cagcttgctc
15661 gtctacttcg tcttcgttgt catcgtcctc ttcttctagg tttatttcaa gagcagtagc
15721 agcttcatcc cattcgcaag gatttggggc acccttggga gccagggcgt tgtaggcagt
15781 gccagagtag ggcttaaaag tagggcccct gtccagcacg ccgcggatgt caaagtacgt
15841 ggaagccatg tccagcacac ggttatcacc cacagctagg gtgaaccgcg ccttgtacga
15901 gtacgcagta tcctcacggt ccacagggat gaaccgcagc gtcaaacgct gggaccggtc
15961 tgtggtcacg tcgtgcgtag gcgccaccgt ggggtttcta aacttgttat tcaggctgaa
16021 gtacgtctcg gtggcgcggg caaactgcac cagcccgggg ctcaggtact ccgaggcgtc
16081 ctggcccgag atgtgcatgt aagaccactg cggcatcatc gaaggggtag ccatcttgga
16141 aagcgggcgc gcggcggctc agcagctcct ctggcggcga catggacgca tacatgacac
16201 acatacgaca cgttagctat cagaagcatc gtcggcgctt cagggattgc acccccagac
16261 ccacgatgct gttcagtgtg ctttgccagt tgccactggc tacgggccgc aacgatcgcg
16321 gaccgctggc ggcgcggcgc agggacgcgc ggctaggacg ggttacaaca acggcggtcg
16381 ggcctggcag cacaggtttc tgctgggtgt cggcgggggg aggcaggtcc agcgttacgg
16441 gtgtgtgctg gcccagcact ccggtagcca tgggcgcgat gggacgggtg gtgggcaggc
16501 cttgctttag tgcctcctcg tacgagggag gctcgtctat ttgcgtcacc agagtttctt
16561 ccctgtcggg gcgcggacgc ttttcgccac gcccctctgg agacactgtc tccacggccg
16621 gtggaggctc ctctacggga gggcggggat caagcttact gttaatctta ttttgcactg
16681 cctggttggc caggtccacc accccgctaa tgccagaggc caggccatct accacctttt
16741 gttggaaatt ttgctctttc aacttatccc tcagcatctg gcctgtgctg ctgttccagg
16801 ccttgctgcc atagttctta acggtggaac cgaaattttt aatgccgctc cacagcgagc
16861 cccagctgaa ggcgccaccg ctcatattgc tggtgccgat atcttgccag tttcccatga
16921 acgggcgcga gccgtgtcgc ggggccagag acgcaaagtt gatgtcttcc attctacaaa
16981 atagttacag gaccaagcga gcgtgagagt ccagactttt tattttgatt tttccacatg
17041 caacttgttt ttaatcagtg tctctgcgcc tgcaaggcca cggatgcaat tccgggcacg
17101 gcgccaatcg ccgcggcgat cagtggaata aggaggggca ggataccgcc gcgcatgcga
17161 cggtgcgacg cgcgccgccg ccggtggtgc gcacgacgca tgccgcccgt caggccgtgg
17221 ccggccatgc ccctcctacg gtgcattctt cctcggaatc ccggcaccgg gaaacggagg
17281 cggcaggtga gggccatatc tgcaagaacc acaaagaccg gcttttaaac gatgctgggg
17341 tggtagcgcg ctgttggcag caccagggtc ctgcctcctt cgcgagccac cctgcgcacg
17401 gaaatcgggg ccagcacggg ctggcgacgg cgacggcggc ggcgggttcc agtggtggtt
17461 cggcgtcggg tagttgctcg tcttctgggg cggtaggtgt agccacgata gccgggggta
17521 ggcgcaatgg aaggatgtag ggcatattcg ggcagtagcg cgctggcggc gccgtacttc
17581 ctcgaacggc gcgggcgccg gggggctgaa acgcgaaaca tccacgggtc cgtttgcacc
17641 tccgtagagg tcttggacgc ggccgcagcg accgcctgca ccgcggcatc cgccaccgct
17701 gaggcaaccg gggacgtttg tgtctccatg ccctctgtgg cggtggcaat actggtgcta
17761 ctggtagtgg gtatctgaac gtccacggtc tgcacgccca gtcccggcgc cacctgcttg
17821 attggccgca cgcggacctc gggctccagc ccaggttcca cggtcatttt ttccaagaca
17881 tcttccagtc gctggcgctt gggtaccatc agctgcacgg tgggtgccaa gtcaccagac
17941 tcgcgcttta ggccgcgctt ttcttcggac ggtgcaagcg cgggcagcac ctgctgcagt
18001 gttacgggct ttaggctagg tgttgggttg ccctcgtcca gcggcaacgc cagcatgtcc
18061 ttatgccgct ttccgtaggc aaactccccg aggcgctcgt tggcctgctc aagcaggtcc
18121 tcgtcgccgt acacctcatc atacacgcgc ttgtaggtgc gggtggagcg ctcaccgggc
18181 gtaaagacta cggtggtgcc gggtcgcaaa acacgtttta cgcgtcgacc tttccactgt
18241 acccgtcgcc tgggcgcggt agcgtgcagc agttccacct cgtcgtcaag ttcatcatca
18301 tcatctttct ttttcttttt gacccgcttt agctttcggg gcttgtaatc ctgctcttcc
18361 ttcttcgggg ggccatagat ctccggcgcg atgacctgga gcatctcttc tttgattttg
18421 cgcttggaca tagcttcgtt gcgcgccgcc gccgctggat acatacaaca gtacgagtct
18481 aagtagtttt ttcttgcaat ctagttgcgc ggggggcggg tgcgcacggg cacgcgcagg
18541 ccgctaaccg agtcgcgcac ccaatacacg ttgcccctgc gaccctgagt catagcacta
18601 atggccgcgg ctgctgcggc ggccgctcgt cgcctggacc tggggggcac agtgacaata
18661 cccgcggcca gccttcgagc ggcccgcatg gccgcccgtc ggccggtgcg acgtgcgcgg
18721 ttaagcaggg ccgccgccgc gcgttgggcg gcagtgccgg gtcggcggcg gtggcgacgt
18781 gctacgcgcc tccgccgtct cttcatttta gcatagcgcc gggctccgcg caccacggtc
18841 tgaatggccg cgtccactgt ggacactggt ggcggcgtgg gcgtgtagtt gcgcgcctcc
18901 tccaccaccg cgtcgatggc gtcatcgacg gtggtgcgcc cagtgcggcc gcgtttgtgc
18961 gcgccccagg gcgcgcggta gtgcccgcgc acgcgcactg ggtgttggtc ggagcgcttc
19021 ttggccccgc caaacatctt gcttgggaag cgcaggcccc agcctgtgtt attgctgggc
19081 gatataagga tggacatgct tgctcaaaaa gtgcggctcg ataggacgcg cggcgagact
19141 atgcccaggg ccttgtaaac gtaggggcag gtgcggcgtc tggcgtcagt aatggtcact
19201 cgctggactc ctccgatgct gttgcgcagc ggtagcgtcc cgtgatctgt gagagcagga
19261 acgttttcac tgacggtggt gatggtgggg gctggcgggc gcgccaaaat ctggttctcg
19321 ggaaagcgat tgaacacgtg ggtcagagag gtaaactggc ggatgagttg ggagtagacg
19381 gcctggtcgt tgtagaagct cttggagtgc acgggcaaca gctcggcgcc caccaccgga
19441 aagttgctga tctggcgcgt ggagcggaag gtcacggggt cttgcatcat gtctggcaac
19501 gaccagtaga cctgctccga gccgcaggtt acgtcaggag tgcaaagcag ggtccatgag
19561 cggattccgg tctgagggtc gccgtagttg tatgcaaggt accagctgcg gtactgggtg
19621 aaggtgctgt cattgcttat taggttgtaa ctgcgtttct tgctgtcctc tgtcaggggt
19681 ttgatcaccg gtttcttctg aggcttctcg acctcgggtt gcgcagcggg ggcggcagct
19741 tcggccgctg cttcggcctc agcgcgcttc tcctcagccc gtgtggcaaa ggtgtcgccg
19801 cgaatggcat gatcgttcat gtcctccacc ggctgcattg ccgcggctgc cgcgttggag
19861 ttctcttccg cgccgctgcc actgctgttg ctgccgcctg cgccaccccc gccctgttcg
19921 gtgtcatctt tcaagctcgc ctggtaggcg tccacatcca acagtgcggg aatgttacca
19981 ccctccagat catcgtaggt gatcctaaag ccctcctgga agggttgccg cttgcggatg
20041 cccaacaagt tgctcaggcg gctgtgggtg aagtccaccc cgcatcctgg cagcaaaatg
20101 atgtctggat ggaaggcttc gtttgtatat accccaggca tgacaagacc agtgacgggg
20161 tcaaacccca gtctgaagtt gcgggtgtca aactttaccc cgatgtcgct ttccagaacc
20221 ccgttctgtc tgcccacttt caagtagtgc tccacgatcg cgttgttcat aaggtctatg
20281 gtcatggtct cggagtagtt gccctcgggc agcgtgaact ccacccactc gtatttcagc
20341 tccacctgat tgtccttagt aggcaagcgc gacaccatca cccgcgcctt aaacttattg
20401 gtaaacatga actcgttcac atttggcatg ttggtatgca ggatggtttt caggtcgccg
20461 ccccagtgcg accggtcgtc aagattgatg gtctgtgtgc ttgcctcccc cgggctgtag
20521 tcattgtttt gaatgaccgt ggtcagaaag ttgctgtggt cgttctggta gttcagggat
20581 gccacatccg ttgacttgtt gtccaccagg tacacacggg tggtgtcgaa taggggtgcc
20641 aactcagagt aacggatgct gtttctcccc ccggtaggcc gcaggtaccg cggaggcaca
20701 aacggcgggt ccaggggagc atcgaaggga gaacccagcg ccgccgccac tggcgccgcg
20761 ctcaccacac tctcgtagga gggaggagga ccttcctcat acatcgccgc gcgccgcata
20821 ctaaggggaa tacaagaaaa ccaacgctcg gtgccatggc cttggtgagt tttttatttt
20881 gcatcatgct tttttttttt taaaacattc tccccagcct ggggcgaagg tgcgcaaacg
20941 ggttgccact ccctcccaaa tccaggacgc tgctgtcgtc tgccgagtca tcgtcctccc
21001 acaccagacc ccgctgacgg tcgtgccttt gacgacgggt gggcgggcgc gggcctggca
21061 cgtccctgtg ctcctgcgcg tacgtcttcc atctactcat cttgtccact aggctctcta
21121 tcccgttgtt gggaaatgcc ggaggcaggt ttttttcgcg ctgcggctgc agcagcgagt
21181 tgtttaggta ctcctcctcg cccagcaggc gcgggcgggt ggtgcgagtg ctggtaagag
21241 accctatcaa gcttggaaat gggctactag catctgaccg cggggccgca gcgcctagat
21301 cggacaagct gcttggcctg cggaagcttt cctttcgcag cgccgcctct gcctgctcgc
21361 gctgttgcaa ctctagcagg gtctgcggtt gcggggaaaa cacgctgtcg tctatgtcgt
21421 cccagaggaa tccatcgtta ccctcgggca cctcgaatcc cccggtgtag aaaccagggg
21481 gcggtagcca gtgcgggttc aagatggcat tggtgaaata ctcggggttc acggcggccg
21541 cgcgatgcaa gtagtccatt aggcggttga taaacggccg gtttgaggca tacatgcccg
21601 gttccatgtt gcgcgcggtc atgtccagcg ccacgctggg cgttaccccg tcgcgcatca
21661 ggttaaggct cacgctctgc tgcacgtagc gcaaaatgcg ctcctcctcg ctgtttaaac
21721 tgtgcaacga ggggatcttc tgccgccggt tggtcagcag gtagtttagg gttgcctcca
21781 ggctgcccgt gtcctcctgc cccagcgcgc ggctgacact tgtaatctcc tggaaagtat
21841 gctcgtccac atgcgcctga cctatggcct cgcggtacag tgtcagcaag tgacctaggt
21901 atgtgtcccg ggacacgctg ccactgtccg tgaagggcgc tattagcagc agcaacaggc
21961 gcgagttggg cgtcagcaag ctagacacgg tcgcgcggtc gcctgtggga gcccgcaccc
22021 cccacagccc ctgcaagttt ttgaaagcct ggctcaggtt tacggtctgc aggccttgtc
22081 tactggtctg gaaaaaatag tctggcccag actggtacac ctcactttgc ggtgtctcag
22141 tcaccattag ccgcagtgcg ctcacaaagt tggtgtagtc ctcctgtccc cgcggcacgt
22201 tggcgggctg tgtactcagg aaggcgttta gtgcaaccat ggagcccagg ttgccctgct
22261 gctgcgcgcg ctcacgctgc gccacggcct cgcgcacatc ccccaccagc cggtccaggt
22321 tggtctgcac gttgccgctg ttgtaacgag ccacgcgctg aagcagcgcg tcgtagacca
22381 ggccggcctc gtcgggccgg atggccctgt tttcggccag cgcgtttacg atcgccagca
22441 ccttctcgtg cgtggggttt gcgcgcgccg ggaccaccgc ttccagaatt gcggagagcc
22501 ggttggcctg cggctgctgc cggaacgcgt caggattgcg cgcagtcagc gacatgatgc
22561 ggtccatgac ctggcgccag tcgtccgtgg agttaaggcc ggacggctgg ctctgcagcg
22621 ccgcccgcac cgccgggtcc gttgcgtctt gcatcatctg atcagaaaca tcaccgctta
22681 gtactcgccg tcctctggct cgtactcatc gtcctcgtca tattcctcca cgccgccgac
22741 gttgccagcg cgcgcgggtg ccaccgccag cccaggtccg gccccagctg cctccagggc
22801 gcgtcggctt ggggcccagc gcaggtcagc gcccgcgtca aagtaggact cggcctctct
22861 atcgccgctg cccgtgccag ccagggccct ttgcaggctg tgcatcagct cgcggtcgct
22921 gagctcgcgc cgccggctca cgctcacggc cttgtggatg cgctcgttgc gataaacgcc
22981 caggtcgtcg ctcaaggtaa gcaccttcag cgccatgcgc atgtagaacc cctcgatctt
23041 tacctccttg tctatgggaa cgtaaggggt atggtatatc ttgcgggcgt aaaacttgcc
23101 caggctaagc atggaatagt tgatggcggc caccttgtca gccaggctca agctgcgctc
23161 ctgcaccact atgctctgca ggatgtttat caaatcgagc agccagcggc cctcgggctc
23221 tactatgttt agcagcgcat ccctgaatgc ctcgttgtcc ctgctgtgct gcactataag
23281 gaacagctgc gccatgagcg gcttgctatt tgggttttgc tccagcgcgc ttacaaagtc
23341 ccacagatgc atcagtccta tagccacctc ctcgcgcgcc acaagcgtac gcacgtggtt
23401 gttaaagctt ttttgaaagt taatctcctg gttcaccgtc tgctcgtatg cggttaccag
23461 gtcggcggcc gccacgtgtg cgcgcgcggg actaatcccg gttcgcgcgt cgggctcaaa
23521 gtcctcctcg cgcagcaacc gctcgcgatt caggccatgc cgcagctcgc gccctgcgtg
23581 gaactttcga tcccgcatct cctcgggctc ctctccctcg cggtcgcgaa acaggttctg
23641 ccgcggcacg tacgcctcac gcgtatcacg cttcagctgc acccttgggt gccgctcagg
23701 agagggcgct cctagccgcg ccaggccctc gccctcctcc aagtccaggt agtgccgggc
23761 ccggcgccgc gggggttcgt aatcaccatc tgctgccgcg tcaaccgcgg atgtcgcccc
23821 tcctgacgcg gtaggaggag gggagggtgc cctgcatgtc tgccgctgct cttgctcttg
23881 ccgctgctga ggaggggggc gcatctgccg cagcaccgga tgcatctggg aaaagcaaaa
23941 aaggggctcg tccctgtttc cggaggaatt tgcaagcggg gtcttgcatg acggggaggc
24001 aaacccccgt tcgccgcagt ccggccggtc cgagactcga accgggggtc ccgcgactca
24061 acccttggaa aataaccctc cggctacagg gagcgagcca cttaatgctt tcgctttcca
24121 gcctaaccgc ttacgctgcg cgcggccagt ggccaaaaaa gctagcgcag cagccgccgc
24181 gcctggaagg aagccaaaag gagcactccc ccgttgtctg acgtcgcaca cctgggttcg
24241 acacgcgggc ggtaaccgca tggatcacgg cggacggccg gatacggggc tcgaaccccg
24301 gtcgtccgcc atgataccct tgcgaattta tccaccagac cacggaagag tgcccgctta
24361 caggctctcc ttttgcacgc tagagcgtca acgattgcgc gcgcctgacc ggccagagcg
24421 tcccgaccat ggagcacttt ttgccgctgc gcaacatctg gaaccgcgtc cgcgactttc
24481 cgcgcgcctc caccaccgcc gccggcatca cctggatgtc caggtacatc tacggatatc
24541 atcgccttat gttggaagat ctcgcccccg gagccccggc caccctacgc tggcccctct
24601 accgccagcc gccgccgcac tttttggtgg gataccagta cctggtgcgg acttgcaacg
24661 actacgtatt tgactcgagg gcttactcgc gtctcaggta caccgagctc tcgcagccgg
24721 gtcaccagac cgttaactgg tccgttatgg ccaactgcac ttacaccatc aacacgggcg
24781 cataccaccg ctttgtggac atggatgact tccagtctac cctcacgcag gtgcagcagg
24841 ccatattagc cgagcgcgtt gtcgccgacc tagccctgct tcagccgatg aggggcttcg
24901 gggtcacacg catgggagga agagggcgcc acctacggcc aaactccgcc gccgccgcag
24961 cgatagatgc aagagatgca ggacaagagg aaggagaaga agaagtgccg gtagaaaggc
25021 tcatgcaaga ctactacaaa gacctgcgcc gatgtcaaaa cgaagcctgg ggcatggccg
25081 accgcctgcg cattcagcag gccggaccca aggacatggt gcttctgtcg accatccgcc
25141 gtctcaagac cgcctacttt aattacatca tcagcagcac ctccgccaga aacaaccccg
25201 accgccgccc gctgccgccc gccacggtgc tcagcctacc ttgcgactgt gactggttag
25261 acgcctttct cgagaggttt tccgatccgg tcgatgcgga ctcgctcagg tccctcggcg
25321 gcggagtacc tacacaacaa ttgttgagat gcatcgttag cgccgtatcc ctgccgcatg
25381 gcagcccccc gccaacccat aaccgggaca tgacgggcgg cgtcttccaa ctgcgccccc
25441 gcgagaacgg ccgcgccgtc accgagacca tgcgccgtcg ccgcggggag atgatcgagc
25501 gctttgtcga ccgcctcccg gtgcgccgtc gtcgccgccg tgtcccccct cccccaccgc
25561 cgccagaaga agaagaaggg gaggccctta tggaagagga gattgaagaa gaagaagagg
25621 cccctgtagc ctttgagcgc gaggtgcgcg acactgtcgc cgagctcatc cgtcttctgg
25681 aggaggagtt aaccgtgtcg gcgcgcaact cccagttttt caacttcgcc gtggacttct
25741 acgaggccat ggagcgcctt gaggccttgg gggatatcaa cgaatccacg ttgcgacgct
25801 gggttatgta cttcttcgtg gcagaacaca ccgccaccac cctcaactac ctctttcagc
25861 gcctgcgaaa ctacgccgtc ttcgcccggc acgtggagct caatctcgcg caggtggtca
25921 tgcgcgcccg cgatgccgaa gggggcgtgg tctacagccg cgtctggaac gagggaggcc
25981 tcaacgcctt ctcgcagctc atggcccgca tttccaacga cctcgccgcc accgtggagc
26041 gagccggacg cggagatctc caggaggaag agatcgagca gttcatggcc gagatcgcct
26101 atcaagacaa ctcaggagac gtgcaggaga ttttgcgcca ggccgccgtc aacgacaccg
26161 aaattgattc tgtcgaactc tctttcaggt tcaagctcac cgggcccgtc gtcttcacgc
26221 agaggcgcca gattcaggag atcaaccgcc gcgtcgtcgc gttcgccagc aacctacgcg
26281 cgcagcacca gctcctgccc gcgcgcggcg ccgacgtgcc cctgccccct ctcccggcgg
26341 gtccggagcc ccccctacct ccgggggctc gcccgcgtca ccgcttttag atgcatcatc
26401 caaggacacc cccgcggccc accgcccgcc gcgcggtacc gtagtcgcgc cgcggggatg
26461 cggcctcttg caagccatcg acgccgccac caaccagccc ctggaaatta ggtatcacct
26521 ggatctagcc cgcgccctga cccgtctatg cgaggtaaac ctgcaggagc tcccgcctga
26581 cctgacgccg cgggagctcc agaccatgga cagctcccat ctgcgcgatg ttgtcatcaa
26641 gctccgaccg ccgcgcgcgg acatctggac tttgggctcg cgcggcgtgg tggtccgatc
26701 caccgtaact cccctcgagc agccagacgg tcaaggacaa gcagccgaag tagaagacca
26761 ccagccaaac ccgccaggcg aggggctcaa attcccactc tgcttccttg tgcgcggtcg
26821 tcaggtcaac ctcgtgcagg atgtacagcc cgtgcaccgc tgccagtact gcgcacgttt
26881 ttacaaaagc cagcacgagt gttcggcccg tcgcagggac ttctactttc accacatcaa
26941 tagccactcc tccaattggt ggcgggagat ccagttcttc ccgatcggct cgcatcctcg
27001 caccgagcgt ctctttgtca cctacgatgt agagacctat acttggatgg gggcctttgg
27061 gaagcagctc gtgcccttca tgctggtcat gaagttcggc ggagatgagc ctctagtgac
27121 tgccgcgcga gacctagccg cgaaccttgg atgggaccgc tgggaacaag acccgcttac
27181 cttctactgc atcaccccag aaaaaatggc cataggtcgc cagtttagga cctttcgcga
27241 ccacctgcaa atgctaatgg cccgtgacct gtggagctca ttcgtcgctt ccaaccctca
27301 tcttgcagac tgggcccttt cagagcacgg gctcagctcc cctgaagagc tcacctacga
27361 ggaacttaaa aaattgcctt ccatcaaggg catcccgcgc ttcttggaac tttacattgt
27421 gggccacaac atcaacggct ttgacgagat cgtgctcgcc gcccaggtaa ttaacaaccg
27481 ttccgaggtg ccgggaccct tccgcatcac acgcaacttt atgcctcgcg cgggaaagat
27541 actcttcaac gatgtcacct tcgccctgcc aaatccgcgt tccaaaaagc gcacggactt
27601 tttgctctgg gagcagggcg gatgcgacga cactgacttc aaataccagt acctcaaagt
27661 catggtcagg gacacctttg cgctcaccca cacctcgctc cggaaggccg cgcaggcata
27721 cgcgctaccc gtagaaaagg gatgctgcgc ctaccaggcc gtcaaccagt tctacatgct
27781 aggctcttac cgttcggagg ccgacgggtt tccgatccaa gagtactgga aagaccgcga
27841 agagtttgtc ctcaaccgcg agctgtggaa aaaaaaggga caggataagt atgacatcat
27901 caaggaaacc ctggactact gcgccctaga cgtgcaggtc accgccgagc tggtcaacaa
27961 gctgcgcgac tcctacgcct ccttcgtgcg tgacgcggta ggtctcacag acgccagctt
28021 caacgtcttc cagcgtccaa ccatatcatc caactcacat gccatcttca ggcagatagt
28081 cttccgagca gagcagcccg cccgtagcaa cctcggtccc gacctcctcg ctccctcgca
28141 cgaactatac gattacgtgc gcgccagcat ccgcggtgga agatgctacc ctacatatct
28201 tggaatactc agagagcccc tctacgttta cgacatttgc ggcatgtacg cctccgcgct
28261 cacccacccc atgccatggg gtcccccact caacccatac gagcgcgcgc ttgccgcccg
28321 cgcatggcag caggcgctag acttgcaagg atgcaagata gactacttcg acgcgcgcct
28381 gctgcccggg gtctttaccg tggacgcaga ccccccggac gagacgcagc tagaccccct
28441 accgccattc tgctcgcgca agggcggccg cctctgctgg accaacgagc gcctacgcgg
28501 agaggtagcc accagcgttg accttgtcac cctgcacaac cgcggttggc gcgtgcacct
28561 ggtgcccgac gagcgcacca ccgtctttcc cgaatggcgg tgcgttgcgc gcgaatacgt
28621 gcagctaaac atcgcggcca aggagcgcgc cgatcgcgac aaaaaccaaa ccctgcgctc
28681 catcgccaag ttgctgtcca acgccctcta cgggtcgttt gccaccaagc ttgacaacaa
28741 aaagattgtc ttttctgacc agatggatgc ggccaccctc aaaggcatca ccgcgggcca
28801 ggtgaatatc aaatcctcct cgtttttgga aactgacaat cttagcgcag aagtcatgcc
28861 cgcttttcag agggagtact caccccaaca gctggccctc gcagacagcg atgcggaaga
28921 gagtgaggac gaacgcgccc ccaccccctt ttatagcccc ccttcaggaa cacccggtca
28981 cgtggcctac acctacaaac caatcacctt ccttgatgcc gaagagggcg acatgtgtct
29041 tcacaccctg gagcgagtgg accccctagt ggacaacgac cgctacccct cccacttagc
29101 ctccttcgtg ctggcctgga cgcgagcctt tgtctcagag tggtccgagt ttctatacga
29161 ggaggaccgc ggaacaccgc tcgaggacag gcctctcaag tctgtatacg gggacacgga
29221 cagccttttc gtcaccgagc gtggacaccg gctcatggaa accagaggta agaaacgcat
29281 caaaaagcat gggggaaacc tggtttttga ccccgaacgg ccagagctca cctggctcgt
29341 ggaatgcgag accgtctgcg gggcctgcgg cgcggatgcc tactccccgg aatcggtatt
29401 tctcgcgccc aagctctacg ccctcaaaag tctgcactgc ccctcgtgcg gcgcctcctc
29461 caagggcaag ctgcgcgcca agggccacgc cgcggagggg ctggactatg acaccatggt
29521 caaatgctac ctggccgacg cgcagggcga agaccggcag cgcttcagca ccagcaggac
29581 cagcctcaag cgcaccctgg ccagcgcgca gcccggagcg caccccttca ccgtgaccca
29641 gactacgctg acgaggaccc tgcgcccgtg gaaagacatg accctggccc gtctggacga
29701 gcaccgacta ctgccgtaca gcgaaagccg ccccaacccg cgaaacgagg agatatgctg
29761 gatcgagatg ccgtagagca ggtgaccgag ctgtgggacc gcctggaact gcttggtcaa
29821 acgctcaaaa gcatgcctac ggcggacggt ctcaaaccgt tgaaaaactt tgcttccttg
29881 caagaactgc tatcgctggg cggcgagcgc cttctggcgg atttggtcag ggaaaacatg
29941 cgagtcaggg acatgcttaa cgaagtggcc cccctgctca gggatgacgg cagctgcagc
30001 tctcttaact accagttgca gccggtaata ggtgtgattt acgggcccac cggctgcggt
30061 aagtcgcagc tgctcaggaa cctgctttct tcccagctga tctcccctac cccggaaacc
30121 gttttcttca tcgccccgca ggtagacatg atccccccat ctgaactcaa agcgtgggaa
30181 atgcaaatct gtgagggtaa ctacgcccct gggccggatg gaaccattat accgcagtct
30241 ggcaccctcc gcccgcgctt tgtaaaaatg gcctatgacg atctcatcct ggaacacaac
30301 tatgacgtta gtgatcccag aaatatcttc gcccaggccg ccgcccgtgg gcccattgcc
30361 atcattatgg acgaatgcat ggaaaatctt ggaggtcaca agggcgtctc caagttcttc
30421 cacgcatttc cttctaagct acatgacaaa tttcccaagt gcaccggata cactgtgctg
30481 gtggttctgc acaacatgaa tccccggagg gatatggctg ggaacatagc caacctaaaa
30541 atacagtcca agatgcatct catatcccca cgtatgcacc catcccagct taaccgcttt
30601 gtaaacactt acaccaaggg cctgcccctg gcaatcagct tgctactgaa agacattttt
30661 aggcaccacg cccagcgctc ctgctacgac tggatcatct acaacaccac cccgcagcat
30721 gaagctctgc agtggtgcta cctccacccc agagacgggc ttatgcccat gtatctgaac
30781 atccagagtc acctttacca cgtcctggaa aaaatacaca ggaccctcaa cgaccgagac
30841 cgctggtccc gggcctaccg cgcgcgcaaa acccctaaat aaagacagca agacacttgc
30901 ttgatccaaa tccaaacaga gtctggtttt ttatttatgt tttaaaccgc attgggaggg
30961 gaggaagcct tcagggcaga aacctgctgg cgcagatcca acagctgctg agaaacgaca
31021 ttaagttccc gggtcaaaga atccaattgt gccaaaagag ccgtcaactt gtcatcgcgg
31081 gcggatgaac gggaagctgc actgcttgca agcgggctca ggaaagcaaa gtcagtcaca
31141 atcccgcggg cggtggctgc agcggctgaa gcggcggcgg aggctgcagt ctccaacggc
31201 gttccagaca cggtctcgta ggtcaaggta gtagagtttg cgggcaggac ggggcgacca
31261 tcaatgctgg agcccatcac attctgacgc accccggccc atgggggcat gcgcgttgtc
31321 aaatatgagc tcacaatgct tccatcaaac gagttggtgc tcatggcggc ggcggctgct
31381 gcaaaacaga tacaaaacta cataagaccc ccaccttata tattctttcc cacccttaag
31441 ctagtttcga aatcgattct agtttcgaag ctgtcgaaga attcgtagct agacaggtgg
31501 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
31561 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
31621 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
31681 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
31741 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
31801 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
31861 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
31921 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
31981 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
32041 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
32101 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
32161 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
32221 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
32281 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
32341 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
32401 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
32461 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
32521 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
32581 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
32641 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
32701 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
32761 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
32821 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
32881 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
32941 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
33001 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
33061 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
33121 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
33181 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
33241 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
33301 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
33361 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
33421 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccg
//
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